The volumes provided are sufficient for preparation of up to 24 reactions (NEB# E7330S) and 96 reactions (NEB# E7330L). Please note that only one index primer is included in this kit. Libraries made with Index 1 cannot be pooled and run together.
If index primers for Pippin Prep The Pippin Prep facilitates library construction for the most popular NGS platforms, and is recommended by Illumina and Ion Torrent for certain workflows.
The platform features the ability to collect narrow and even fragment distributions, as well as the flexibility to collect wide ranges of fragments with minimal effort. This effect can be completely overcome by using a time ramp whose longest reverse time is long enough to separate all molecules of interest. 5 Pippin Pulse User Manual Rev I 3.
0 Using the Pippin Pulse The Pippin Pulse software includes five preset protocols based on internal testing. Pippin Prep (Cat# PIP0001) or Blue Pippin (Cat# BLU0001) by SAGE Science Qubit fluorometer (Cat# Q, Thermo Fisher Scientific) qPCR instrument (optional) for Pippin Prep selection: 3 The NEBNext Multiplex Small RNA Library Prep Set for Illumina (Set 1) contains the adaptors, primers, enzymes and buffers required to convert small RNAs into indexed libraries for next generation sequencing on the Illumina platform.
The novel workflow has been optimized to minimized adaptor Marine Biological Laboratory Library Prep Kit for Illumina (Index Primers 148) 2 NEBNext Multiplex Small RNA Library Prep Kit for Illumina (Index Primers 148) Includes (Cont. ): (blue) NEBNext Index 7 Primer for Illumina (blue) NEBNext Index 8 Primer for Illumina for Pippin Prep The Pippin Prep benefits pairedend sequencing methods byimproving the efficiency of runs and the value of the data produced: Includes instrument, US keyboard, mouse, mouse pad, USB stick, rinse cassette and user manual.
Requires monitor (1366 x 768 resolution). Dimensions external HxWxD, mm. 180 x 270 x 530. Dimensions internal HxWxD, mm DNA Size Selection System Operations Manual Software v. 6. 00 Cassette Definition Set 7. www. sagescience. com Pippin Prep Operations Manual software v.
6. 00 CD6 ii Pippin Prep Operations Manual software v. 6. 00 Size Select Up To 24 Samples In One Run PippinHT incorporates the companys highly regarded sizing technology and increases throughput to 24 samples per run, compared to a maximum of five with Pippin Prep or BluePippin. The instrument offers an efficient, automated solution for scientists running busy sequencing labs, core facilities, and other laboratories producing largescale sequencing data.
Pippin Prep Support. Software. PippinPrep Software v6. 31 v6. 40, Cassette Definition Set 12 675KB. User Manuals. Pippin Prep User Manual v6. 23 CD10 2. 9MB updated 9216. Pippin Prep Cassette Reference Chart 623 CD10 371KB. Pippin Prep Installation Guide 407KB. Pippin Prep IQOQ Document 483KB. Cassette Guides. DNA Sequences of Internal Prepare Amplicon Libraries Requiring Fragmentation Using the Ion Xpress Pippin Prep instrument Prepare Amplicon Libraries Requiring Fragmentation Using the Ion Xpress