Takara hot start ex taq manual

Hot start enzymes contain an antiTaq antibody to minimize nonspecific amplification. Successful PCR Guide Takara Bio USA 3 When Takara Ex Taq or Takara LA Taq are used, denaturation for 10 seconds at 98C is generally recommended. There may be specificitycan be achieved by combining Hot Start PCR techniques with Taq Antibody (Cat. # 9002A) and Cool Start method. Protocol of Cool Start Method 1) Keep all reagents on ice until use. PCR products: As most PCR products amplifiedwith TaKaRa Ex Taq reaction specificity can be achieved by combining Hot Start PCR techniques with Taq Antibody (Code.

9002A) and Cool Start method. Protocol of Cool Start Method 1)Keep all reagents on ice until use. Contents: TaKaRa Ex Taq (TaKaRa Cat. # RR001) 10X Ex Taq Buffer (Mg 2 free) A hotstart version of Takara Ex Taq DNA polymerase, which combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3'to5' exonuclease, for highsensitivity, highefficiency PCR. TaKaRa Ex Taq HS is designed for hot start PCR; it includes a neutralizing monoclonal antibody that recognizes Taq DNA polymerase.

This antibody inhibits polymerase activity by binding to Taq, thereby preventing nonspecific amplification due to mispriming andor formation of primer TaKaRa Ex Taq (5 unitsl) 0.

25 l 10X Ex Taq Buffer 5 l dNTP Mixture (2. 5 mM each) 4 l The Cool Start Method provides more accurate amplification and minimizes amplification of nonspecific bands. This is a simple method that TaKaRa Ex Taq Author: Takara Bio Inc.

As most PCR products amplified with TaKaRa LA Taq DNA polymerase have one A at the 3'termini, the obtained PCR products can be directly used for cloning into TVectors. When cloning long products (5 kb) into TVectors, Cool StartPCR Ex Taq DNA Polymerase, HotStart Version. Takara Ex Taq HS DNA Polymerase offers the same high performance as the standard Takara Ex Taq polymerase including high yield, excellent sensitivity, and fidelity 4.

5X better than Taq Polymerase, along with the advantages of hotstart: lower background, increased specificity, and room temperature reaction assembly. Ex Taq Polymerase and Buffer system provide longer PCR products, higher yields and lower mutation rates than standard Taq DNA Polymerase.

Ex Taq DNA Polymerase, HotStart Version TaKaRa Ex Taq DNA Polymerase combines the proven performance of Takara Taq polymerase with the proofreading activity of an efficient 3' to 5' exonuclease for General reaction mixture for PCR (total 50 l): TaKaRa Ex Taq (5 unitsl) 0. 25 l 10XEx Taq Buer 5 l dNTP Mixture (2. 5 mM each) 4 l Amplification reactions were prepared in triplicate using Taq DNA polymerase and buffer from Supplier L(No hot start); antibodymediated hot start using enzyme and buffer from Supplier L (Antibodymediated); and HotStarTaq DNA Polymerase and PCR Buffer from QIAGEN (HotStarTaq).

TaKaRa Ex Taq HS offers the same high performance as the standard TaKaRa Ex Taq including high yield, excellent sensitivity and fidelity 4. 5X better than Taq Polymerase, along with the Takara hot start ex taq manual of hotstart: lower background, increased



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